Orphos is a fast, browser-based implementation of Prodigal for prokaryotic gene prediction. It runs entirely in your web browser using WebAssembly, allowing you to analyze bacterial and archaeal genomes without installing any software.

Is Orphos a replacement for Prodigal?

Yes! Orphos implements the same dynamic programming algorithm as Prodigal and produces compatible output formats.

Is my genomic data sent to a server?

No. Orphos runs 100% locally in your web browser. Your sequences never leave your computer, ensuring complete privacy for sensitive or proprietary genomic data.

What types of genomes can Orphos analyze?

Orphos is designed for prokaryotic genomes, including bacteria and archaea. It supports complete genomes, draft assemblies, plasmids, and metagenomic contigs. It is not suitable for eukaryotic gene prediction.

How do I use Orphos for gene prediction?

Simply upload a FASTA file or paste your sequence, select your analysis mode (single genome or metagenomic), choose an output format (GFF3, GenBank, BED6, SCO, or GCA), and click Analyze. Results appear in seconds and can be downloaded or copied.

Does Orphos work offline?

After the initial page load, Orphos can work offline thanks to Progressive Web App technology. All gene prediction happens locally in your browser without requiring internet connectivity.

Prokaryotic gene prediction in your browser

Fast, private, Prodigal-compatible gene finding powered by Rust and WebAssembly. No installation required.

Input sequence

Choose FASTA file No file chosen

Or paste FASTA sequence

Options

Analysis mode Use metagenomic for short, fragmented sequences

Output format

Translation table

Circular topology (allow wraparound genes)

Closed ends (no genes off edges)

Mask runs of N's

Force non-Shine-Dalgarno models

Prokaryotic gene prediction in your browser

Input sequence

Options

Results

Error